Assemble thousands of samples in minutes!
You have a clone library with 500 clones and you use two primers (Forward and Reverse) to sequence each clone. At the end of sequencing process, you will have a folder with 1000 sequences, which need to be assembled in 500 contigs. It would be rather tedious to assemble a contig at a time. However, DNA Baser is the only software that allows you to assemble all sequences in one-step. The prerequisite is that the sequences that belong to the same contig are:
a. Placed in a separate folder
b. Named after a pattern (in this case see Batch sequence assembly by filename pattern)
Fig.1 - The Project manager
Select the samples to be assembled
Navigate to the folder that contains your samples. Remember that samples that belong to the same contig need to be grouped in their own folders. You don't have to put these samples in the Job List. The sub-folder structure should look like this:
Start the assembly
To start the assembly, just press the START BATCH ASSEMBLY button. DNA Baser will detect the samples belonging to the same contig and assemble them. Don't leave the computer. DNA Baser will finish before you have the time to drink your coffee.
During the batch assembly process, a detailed log is generated. It contains information about each individual assembling process, a batch job summary, the list of parameters used for assembling, quality of each assembly job and many many other statistics.
During batch assembly, DNA Baser creates the following folders (located in current folder):
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