NextGen Workbench

How to merge FastQ files?

A free program for Windows called DiskTools allows us to merge two or more FastQ files with a single click.

1. Download DiskTools from here

2. Go to 'Merge Files' module/tab

3. In the left panel, locate your FastQ files

4. Make sure the 'Separator' box empty (there is no Enter, no space, no tab, no comma, etc)

5. Press 'Merge'

This method will not work with SFF files!